We have examined the involvement of the mitochondrial permeability transition in isolated CNS mitochondria from normal and Huntington's Disease model mice. At the systems level, we are currently examining metabolite changes in brains of Huntington's Disease model mice and how these metabolite changes can be used both as biomarkers for disease progression and to provide insight into mechanisms of disease progression.
A new project is investigating how certain classes of drugs might alter synaptic vesicle availability or cycling as a means of limiting high frequency activity in epilepsy.
Research in Science Teaching:
The field of neuroscience probes the cellular, molecular and circuit properties contributing to learning and memory, processes that should influence teachers’ classroom strategies and practices. Providing professional development for postbaccalaureate teachers into the biological basis of learning and memory applies the new knowledge generated in my field towards solving a pressing societal need, improving K-12 science education. As a research scientist, communicating this knowledge to teacher audiences is a part of translational research. BrainU is a professional development program for K-12 science teachers interested in understanding the brain and its relevance to education (www.brainu.org). BrainU was created in response to requests from teachers to learn more neuroscience than could be covered in one-hour classroom visits. My goals for the BrainU program are to engage teachers both in teaching about and applying neuroscience to their classroom practices and to motivate teachers and their students to learn more about themselves and the pursuit of science. Our research goals are to probe how learning about the nervous system motivates teachers and students to teach, learn and do science in their classrooms.
(For a comprehensive list of recent publications, refer to PubMed, a service provided by the National Library of Medicine.)
Lou S, Lepak VC, Eberly LE, Roth B, Cui W, Zhu XH, Öz G, Dubinsky JM. Oxygen consumption deficit in Huntington disease mouse brain under metabolic stress. Hum Mol Genet. 2016 May 18;.
Yang X, Meehan AL, Rothman SM, Dubinsky JM. Seletracetam enhances short term depression in vitro. Epilepsy Res. 2015 Aug 8;117:17-22.
Abu-Odeh D, Dziobek D, Jimenez NT, Barbey C, Dubinsky JM. Active learning in a neuroethics course positively impacts moral judgment development in undergraduates. J Undergrad Neurosci Educ. 2015 Mar 15;13(2):A110-9.
Fitzakerley JL, Michlin ML, Paton J, Dubinsky JM. Neuroscientists' classroom visits positively impact student attitudes. PLoS One. 2013 Dec 16;8(12):e84035.
Cui W, Zhu XH, Vollmers ML, Colonna ET, Adriany G, Tramm B, Dubinsky JM, Öz G. Non-invasive measurement of cerebral oxygen metabolism in the mouse brain by ultra-high field (17)O MR spectroscopy. J Cereb Blood Flow Metab. 2013 Dec;33(12):1846-9.
Dubinsky JM, Roehrig G, Varma S. Infusing Neuroscience into Teacher Professional Development. Educ Res. 2013 Aug-Sep;42(6):317-329.
Roehrig GH, Michlin M, Schmitt L, MacNabb C, Dubinsky JM. Teaching Neuroscience to Science Teachers: Facilitating the Translation of Inquiry-Based Teaching Instruction to the Classroom. Cell Bio Ed/Life Science Ed. 2012 Winter;11(4):413-24.
Zacharoff L, Tkac I, Song Q, Tang C, Bolan PJ, Mangia S, Henry PG, Li T, Dubinsky JM. Cortical metabolites as biomarkers in the R6/2 model of Huntington's disease. J Cereb Blood Flow Metab. 2011 Nov 2.
Tkac I, Henry PG, Zacharoff L, Wedel M, Gong W, Deelchand DK, Li T, Dubinsky JM. 2012 Homeostatic adaptations in brain energy metabolism in mouse models of Huntington disease. J Cereb Blood Flow Metab. 2012 Jul 18. doi: 10.1038/jcbfm.2012.104
Dubinsky JM. Neuroscience education for prekindergarten-12 teachers. J Neurosci. 2010 Jun 16;30(24):8057-60.
Dubinsky, JM 2009 Heterogeneity of Nervous System Mitochondria; Location, Location, Location! Experimental Neurology, 18:293-307
Tkac I, Dubinsky JM, Keene CD, Gruetter R, Low WC. Neurochemical changes in Huntington R6/2 mouse striatum detected by in vivo 1H NMR spectroscopy. J Neurochem. 2007 Mar;100(5):1397-406.
MacNabb C, Schmitt L, Michlin M, Harris I, Thomas L, Chittendon D, Ebner TJ, Dubinsky JM. 2006 Neuroscience in Middle Schools: A Professional Development and Resource Program that Models Inquiry-Based Strategies and Engages Teachers in Classroom Implementation. Cell Biol Educ / Life Sci Educ. 5(2):144-57.
Brustovetsky N, R.LaFrance, K.J. Purl, T. Brustovetsky, C.D. Keene, W.C. Low, and J.M. Dubinsky. 2005 Age-Dependent Changes in the Calcium Sensitivity of Striatal Mitochondria in Mouse Models of Huntington's Disease. J Neurochem. 93:1361-70.
LaFrance, R, N Brustovetsky, C Sherburne, D DeLong, JM Dubinsky, 2005 Age-Related Changes in Regional Brain Mitochondria from Fischer 344 Rats. Aging Cell 4:139-145.
Brustovetsky T., Purl K, Young A, Shimizu K and Dubinsky JM. 2004 Dearth of Glutamate Transporters Contributes to Striatal Excitotoxicity, Exp Neurol 189:222-30.
Dubinsky JM, N Brustovetsky, R LaFrance 2004 Protective Roles of CNS Mitochondria J. Bioeng Biomemb 36:299-302.
Brustovetsky, N., Dubinsky, JM, Antonsson, B., and Jemmerson, R. 2003 Two pathways for tBID-Induced Cytochrome c Release from Rat Brain Mitochondria: BAK- versus BAX-Dependence. J Neurochem 84:196-207.
Brustovetsky N, Brustovetsky T, Purl KJ, Capano M, Cromton M and Dubinsky JM 2003 Increased Susceptibility of Striatal Mitochondria to Calcium-Induced Permeability Transition. J. Neurosci. 23:4858-67.