Adenosine-evoked potassium currents in retinal ganglion cells
Assistant Director, Institutional Animal Care and Use Committee (IACUC), Office of the Vice President for Research, University of Minnesota - Twin Cities
Eric Newman, Ph.D.
Previously, our lab has shown that glial cells in the retina modulate retinal ganglion cell activity by releasing ATP. ATP in the intracellular space is metabolized to adenosine which leads to the hyperpolarization of retinal ganglion cells. Using calcium imaging and whole-cell electrophysiology we have investigated the cellular mechanisms that cause the adenosine-evoked hyperpolarization and consequent altered spiking of retinal ganglion cells. To determine the intracellular mechanisms mediating the adenosine response, we developed a double patch clamp technique. This technique allows us to acquire control recordings from the cell and then later manipulate that cell's intracellular signaling cascades by introducing reagents through a second patch electrode. We have found that there are two adenosine-evoked currents in retinal ganglion cells. One that is mediated by G-protein gated inward rectifying K + channels and another that is mediated by small conductance Ca 2+ -activated K + channels.
- Paulo Kofuji
- Mark Thomas
- Eric Newman
Courses Taken Beyond the Core Courses:
- NSC 5201
- NSC 5202
- PHCL 5110
- PSY 8960
- PSY 5960
- PSY 5031
Conferences Attended and Presentations:
- Society for Neuroscience Annual Meeting – 2003, 2005, 2006, 2007, 2008
- Paulo Kofuji (chair)
- Bob Miller
- Dwight Burkhardt
- Eric Newman (advisor)
- Clark BD, Kurth-Nelson ZL, Newman EA. Adenosine-evoked hyperpolarization of retinal ganglion cells is mediated by G-protein-coupled inwardly rectifying K+ and small conductance Ca2+-activated K+ channel activation. J Neurosci. 2009;29:11237-11245.
Awards and Honors:
- NSF Vision Training Grant (2003- 2007)
- Society for Neuroscience